Date Received: 08-01-2026 / Date Accepted: 12-05-2026 / Date Published: 27-05-2026
Actinobacillus pleuropneumoniae is the causative agent of contagious pleuropneumonia in pigs, resulting in significant economic losses to the swine industry, while the increasing prevalence of antibiotic resistance has reduced the effectiveness of treatment strategies. This study aimed to isolate and identify A. pleuropneumoniae from pigs suspected of respiratory disease and to evaluate the antibiotic susceptibility profiles of the isolated strains from commercial pig farms in Hung Yen Province and Hanoi City, Vietnam. Lung tissue samples were collected from 52 pigs showing clinical signs of Pleuropneumonia. Bacterial isolation was performed on blood agar supplemented with nicotinamide adenine dinucleotide (NAD), followed by identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) in combination with biochemical tests. Minimum inhibitory concentrations (MICs) were determined by the agar dilution method for eight antibiotics commonly used in veterinary practice. The results showed that A. pleuropneumoniae was isolated from 17 out of 52 samples (32.69%). High resistance rates were observed for tetracycline (88.24%), ampicillin (70.59%), and florfenicol (64.71%), whereas the isolates remained highly susceptible to tiamulin. Overall, 58.82% of the isolates were classified as multidrug-resistant, exhibiting various resistance phenotypes. These findings highlight the urgent need for stringent control measures on antibiotic use in livestock production to mitigate the emergence and spread of antimicrobial resistance and to improve therapeutic outcomes.